Reflective Practive

ImmunoCAP

ImmunoCAP tests, designed to run on Phadia Laboratory Systems, give reliable results that support primary care physicians as well as specialists in providing optimal patient management.

How do they work?

Designed as sandwich assays, the solid phase of ImmunoCAP ensures binding of relevant antibodies. This is achieved through an extreme binding capacity in combination with an optimal amount of cellulose in each ImmunoCAP. The solid phase coupled to our standardised allergen source material, recombinant allergen components and relevant markers ensures high performance and reliable results.

The allergen component on the solid phase reacts with the specific IgE in the patient serum sample.

After washing away non-specific IgE, enzyme-labelled antibodies against IgE are added to form a complex.

After incubation, unbound enzyme-labelled anti-IgE is washed away and the bound complex is then incubated with a developing agent.

The higher the fluorescence, the more specific IgE is present in the sample.

ImmunoCAP Specific IgE detects IgE antibodies in the range 0 to 100 kUA/L, where A represents allergen-specific antibodies. The result is reported quantitatively. 0.1 kUA /L is the lower detection limit. A large number of studies have been performed in which the clinical performance of ImmunoCAP Specific IgE tests in allergy diagnosis have been evaluated. Clinical performance is expressed as sensitivity, ranging from 84-95%, and specificity, ranging from 85-94%. Sensitivity and specificity have been reported from multicentre studies including several hundred patients tested for a range of different allergens.

Are you a laboratory professional?

This educational website, provided by Thermo Fisher Scientific, ImmunoDiagnostics Division, has been produced in partnership with the Institute of Biomedical Science as a pioneering venture to promote excellence in laboratory practice using education and continuing professional development (CPD) resources.

The information contained within this website is intended for laboratory professionals.

By entering this website you are confirming that you are a laboratory professional.

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