ImmunoCAP ISAC is a highly advanced tool for revealing the patient's IgE antibody profile. It is the result of a combination of innovative biochip technology and cutting edge research in molecular allergology. It is the only in vitro diagnostic test for simultaneous measurement of specific IgE antibodies to a broad spectrum of allergen components.
How do they work?
The biochip technology takes the form of a miniaturised immunoassay platform where allergen components are immobilised in a microarray. This enables measurement of IgE antibodies to a fixed panel of 112 components from 51 allergen sources in a single step, using only 30 μl of serum or plasma. The allergen components are spotted in triplets and covalently immobilised on a polymer coated slide. Each slide contains four microarrays giving results for four different samples per slide.
ImmunoCAP ISAC is a two-step assay:
IgE antibodies from the patient sample bind to the immobilised allergen components
Allergen-bound IgE antibodies are detected by a fluorescence labelled anti-IgE antibody
For each patient's allergens ImmunoCAP ISAC Xplain answers the following questions:
- Is the molecule species-specific or cross-reactive?
- Is the molecule stable and therefore also prone to elicit reactions to heated food?
- Is the molecule commonly associated with no (asymptomatic), local or systemic reactions?